Hoffman Modulation Contrast Microscopy

Section Overview:

Hoffman modulation contrast is an oblique illumination technique that enhances contrast in both stained and unstrained specimens by detection of optical phase gradients. This section includes discussions of transmitted and reflected light applications using Hoffman modulation contrast and links to interactive Java tutorials designed to aid in understanding the technique. Also included are virtual microscopes and an image gallery of photomicrographs made using modulation contrast either alone or in combination with other illumination mechanisms.

Review Articles

Interactive Tutorials

Digital Image Gallery

Hoffman Modulation Contrast Image Gallery

Modulation contrast illumination produces a pseudo three-dimensional effect that can be used in combination with other illumination techniques to achieve spectacular images.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Kirill I. Tchourioukanov and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.