Tahr Ovary Epithelial Cells (HJ1.Ov Line

Tahr Ovary Epithelial Cells (HJ1.Ov Line)

Applying a popular triple fluorophore combination, the adherent log phase culture of HJ1.Ov cells illustrated in this section was treated with MitoTracker Red CMXRos in growth medium for one hour, washed and fixed with paraformaldehyde (prepared in growth medium), permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with Oregon Green 488 conjugated to phalloidin and counterstained with Hoechst 33258.