Darkfield Microscope Configuration

The following section reviews the steps in the configuration and alignment of a microscope for both low and high magnification transmitted darkfield illumination.

Low Magnification Transmitted Darkfield Microscope Configuration

High Magnification Transmitted Darkfield Microscope Configuration

Careful attention should always be given to microscope alignment, irrespective of whether the illumination mode is brightfield, darkfield, phase contrast or some other contrast enhancement technique. Time spent in this endeavor will be repaid in excellent performance of the microscope both for routine observation and critical digital imaging or photomicrography.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.