Condenser and Field Diaphragm Alignment

This tutorial demonstrates how the condenser is centered in the optical path and the size of the field diaphragm opening is determined when adjusting a microscope for proper Köhler illumination. Operating instructions appear just below the tutorial window.

Error processing SSI file

The default initial state of the microscope is severely out of alignment. To operate this tutorial, use the sliders to shift the focus of the sample image and to position both the condenser and the field diaphragm according to the steps below:

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.