Cells in Culture: Confocal Microscopy Digital Image Gallery
The Olympus Microscopy Resource Center galleries include images of fluorescent specimens, as well as darkfield, phase contrast, and Hoffman modulation contrast photomicrographs. In addition, the gallery features streaming video and images from featured microscopists.
The clonal A-10 line was established form the thoracic aorta of a DB1X strain embryonic rat (Rattus norvegicus). The morphology of A-10 cells is similar to that of myoblasts, the precursors of muscle fibers. In culture, the cells grow adherently and generate spontaneous action potentials at the stationary phase of the growth cycle. An increase in activity of the enzymes myokinase and creatine phosphokinase in the cells has been documented.
J. Ponten and E. Saksela established the U-2 OS human cell line from a sarcoma of the tibia that was moderately differentiated. Males are more commonly afflicted with osteosarcoma than females, but the patient from whom the original cells were sampled was a fifteen-year-old girl.
From 1966 to 1969, multiple cell lines were established by J. Ponten and associates from human gliomas, including the U-118 MG line. The U-118 MG line, which has been found to be tumorigenic in nude mice inoculated subcutaneously, contains both glioblastoma and astrocytoma cells.
The HeLa cell line, which was initiated from a cervical adenocarcinoma, was the first successful immortal human cell line. Soon after the establishment of the epithelial line in the early 1950s, it was utilized in the development and testing of the polio vaccine. The proliferation of HeLa cells is remarkable, an entire generation of the cells being produced about once every day.