Normal African Green Monkey Kidney Epithelial Cells (Vero Line)

Examining the Proximity Between the Nucleus and the Mitochondrial Network in Monkey Kidney Epithelial Cells

A log phase monolayer culture of Vero cells was treated with MitoTracker Red CMXRos (pseudocolored yellow) in growth medium for one hour, washed, and fixed with 3.7-percent paraformaldehyde in medium containing serum. After washing and permeabilization, the cells were blocked with bovine serum albumen in PBS and labeled with BODIPY FL conjugated to phallacidin. The nuclei were subsequently counterstained with DRAQ5, an anthraquinone that exhibits preferential intercalation at AT base pairs.