Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)

Employing Phallotoxins to Target the F-Actin Network in Monolayer PtK2 Cell Cultures

Filamentous actin was targeted in the culture of rat kangaroo kidney (PtK2) cells presented in this section with BODIPY FL conjugated to phallacidin, a bicyclic peptide derived from a toxic mushroom. In addition, the cells were treated with the mitochondrion-selective stain MitoTracker Red CMXRos and a nuclear counterstain, TO-PRO-3 (pseudocolored cyan).