Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)

Rat Kangaroo Kidney Epithelial Cells with MitoTracker Red CMXRos, BODIPY FL, and DRAQ5

An adherent monolayer culture of male rat kangaroo kidney epithelial cells was treated with MitoTracker Red CMXRos in growth medium for one hour, washed, and fixed with 3.7-percent paraformaldehyde in medium containing serum. After washing and permeabilization, the cells were blocked with bovine serum albumen in PBS and labeled with BODIPY FL conjugated to phallacidin. The nuclei were subsequently counterstained with DRAQ5 (pseudocolored cyan), an anthraquinone that exhibits preferential intercalation at AT base pairs.