Opossum Kidney Epithelial Cells (OK Line)

Visualizing Mitosis in OK Cell Cultures with Immunofluorescence

The OK cell appearing in this section is in the early anaphase stage of mitosis, the chromosomes appearing to have just become completely separated. The chromosomes were visualized by treating the culture that this cell was resident in with mouse anti-histone (pan) monoclonal antibodies, followed by secondary goat anti-mouse antibodies conjugated to Texas Red.