Indian Muntjac Deer Skin Fibroblast Cells

The Mitotic Apparatus in Dividing Indian Muntjac Deer Skin Cells

The first stages of mitosis are revealed in the confocal image of Indian Muntjac deer skin cells presented in this section, which were stained with Alexa Fluor 568 (tubulin) and TO-PRO-3 (DNA). The cells were fixed with 3.7 percent paraformaldehyde, permeabilized with Triton X-100, blocked with 10 percent normal goat serum and treated with mouse anti-alpha-tubulin primary antibodies. Goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568 were mixed with the DNA stain.