Indian Muntjac Deer Skin Fibroblast Cells

Imaging the Mitochondria Network, Filamentous Actin, and Nuclei in Indian Muntjac Deer Skin Fibroblast Cells

A triple fluorophore combination of MitoTracker Deep Red 633, BODIPY FL conjugated to phalloidin, and SYTOX Orange was used to label an adherent log phase culture of Indian Muntjac cells for mitochondria, the filamentous actin network, and nuclear DNA, respectively. The cells were first treated with MitoTracker Deep Red 633 in growth medium for one hour, washed and fixed with paraformaldehyde (prepared in growth medium), permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with the conjugated phalloidin and counterstained with the SYTOX reagent.