Mongolian Gerbil Lung Fibroblast Cells (GeLu Line)

Gerbil Lung Cells Labeled for Tubulin with Immunofluorescence

In order to visualize the microtubules present in the gerbil lung cells shown in this confocal image, a GeLu culture was immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the cells were labeled for the cytoskeletal F-actin network with Alexa Fluor 546 (pseudocolored blue) conjugated to phalloidin, and for cell nuclei with TO-PRO-3.