Swiss Mouse Embryo Moloney Murine Leukemia Virus Transfected Fibroblast Cells (CRE BAG 2 Line)

Targeting Tubulin in CRE BAG 2 Cells with Immunofluorescence

In order to visualize the microtubules present in a culture of transfected Swiss mouse fibroblasts, the cells were immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 555 (pseudocolored blue). In addition, the cells were labeled for DNA in the nucleus with the red-absorbing dye TO-PRO-3.