Transformed African Green Monkey Kidney Fibroblast Cells (COS-7 Line)

Targeting F-Actin in African Green Monkey Kidney Cell Cultures with Phallotoxins

The culture of COS-7 kidney cells presented in this section was labeled for mitochondria with MitoTracker Orange CMTMRos and for the cytoskeletal filamentous actin network with BODIPY FL conjugated to phallacidin, a cyclic peptide derived from a toxic mushroom (Amanita phalloides). In addition, the cells were probed for DNA in the cell nucleus with TO-PRO-3, a monomeric cyanine nucleic acid stain.