Embryonic Albino Swiss Mouse Fibroblast Cells (3T3 Line)

Imaging the Cytoskeletal Network in 3T3 Cell Cultures

The isolated 3T3 cell presented in this section was resident in an adherent culture stained for F-actin with Alexa Fluor 546 conjugated to phalloidin, and for DNA with the red-absorbing dye TO-PRO-3. In addition, the culture was immunofluorescently labeled with Alexa Fluor 488 conjugated to antibodies that target alpha-tubulin, a major component of the microtubule network.