Embryonic Albino Swiss Mouse Fibroblast Cells (3T3 Line)

Targeting Filamentous Actin in Mouse Fibroblasts with a Phallotoxin

A log phase culture of 3T3 cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. In addition, the F-actin network was labeled with Alexa Fluor 633 (pseudocolored blue) conjugated to phalloidin, a phallotoxin isolated from the toxic death cap mushroom.